Journal: bioRxiv

Article Title: Increased 5-HT 2A receptor signalling efficacy differentiates serotonergic psychedelics from non-psychedelics

doi: 10.1101/2024.06.13.594677

Figure Lengend Snippet:

Article Snippet: C6 glioma cells which endogenously express the rat 5-HT 2A receptor , (ATCC CCL-107) were maintained in Ham’s F12 nutrient mix containing 2 mM Glutamax, 10 % (v/v) FBS and 100 I.U. μg - ml - penicillin/streptomycin.

Techniques: Derivative Assay, Expressing

Journal: bioRxiv

Article Title: Psilocybin facilitates fear extinction: importance of dose, context, and serotonin receptors

doi: 10.1101/2024.05.04.592469

Figure Lengend Snippet: (A) Experimental timeline. (B) Fraction of time spent freezing during habituation and different binned CS periods during extinction on day 3. Black, vehicle + vehicle. Red, vehicle + 1 mg/kg psilocybin. Line, mean. Error bars, ± s.e.m.. Shown is the p-value (if <0.05) for between-group post hoc analysis between vehicle + vehicle and vehicle + 1 mg/kg psilocybin performed after generalized linear mixed effects model with fixed factors of antagonist, psilocybin, time, as well as all 2- and 3-way interactions. (C) Similar to (B) for extinction retention on day 4. (D) Similar to (B) for fear renewal on day 11. (E – G) Similar to (B) – (D) for MDL100907 + vehicle (teal) and MDL100907 + psilocybin (purple). Dashed line, the vehicle + vehicle data repeated from (B) – (D) for purpose of comparison. (H – J) Similar to (B) – (D) for WAY100635 + vehicle (yellow) and WAY100635 + psilocybin (orange). Dashed line, the vehicle + vehicle data repeated from (B) – (D) for purpose of comparison. N = 64 including 8 males and 8 females for vehicle + vehicle, 8 males and 8 females for vehicle + psilocybin, and 4 males and 4 females each for MDL100907 + vehicle, MDL100907 + psilocybin, WAY100635 + vehicle, and WAY100635 + psilocybin.

Article Snippet: The 5-HT 2A receptor antagonist MDL100907 (4173, Tocris Biosciences) was dissolved in a 0.9% saline solution containing 0.3% Tween 80 (P1754, Sigma-Aldrich) for a 1 mg/mL stock solution.

Techniques: Comparison